METABOLOMICS

Finding your way through complex metabolic pathways

  • We provide a targeted and untargeted approach for a wide range of metabolites present in your samples using triple quadrupole, Q-TOF or Orbitrap mass spectrometry.


    [/fusion_li_item]
  • We include the necessary sample preparation steps prior to analysis, such as precipitation, liquid-liquid extraction, solid-phase extraction, derivatization.

  • We apply state-of-the-art gas chromatography (GC), liquid chromatography (LC) and capillary electrophoresis (CE) methodologies to tackle the diverse physicochemical properties of metabolites (polar, apolar, volatile, non-volatile, etc.). If methods are not available yet, we go back to the drawing board and develop the method from scratch and verify its performance.

  • We annotate metabolites using an in-house built accurate mass retention time (AMRT) or retention time locked (RTL) library which is continuously being updated with new entries.

  • We perform metabolomics experiments with project-specific QC samples guaranteeing validity of results.

  • We follow a rigorous and established game plan that helps define the right questions and determine which data will provide the answers.

  • [/fusion_checklist]
    [/fusion_builder_column][/fusion_builder_row][/fusion_builder_container]

    A few examples of projects we have supported in the past

    • Effect of genetic modifications on metabolite read-out (knock-out, knock-down, knock-in)

    • Discovery of biomarkers for bacterial infection in cystic fibrosis patients

    • Batch comparisons of complex raw materials and link with final product quality (e.g. fermentations)

    • Monitoring overall progress of chronic kidney disease

    • Early detection of parasite infections in humans

    • Studying the effect of drugs on cell metabolism