METABOLOMICS

Finding your way through complex metabolic pathways

  • We provide a targeted and untargeted approach for a wide range of metabolites present in your samples using triple quadrupole, Q-TOF or Orbitrap mass spectrometry.

  • We include the necessary sample preparation steps prior to analysis, such as precipitation, liquid-liquid extraction, solid-phase extraction, derivatization.

  • We apply state-of-the-art gas chromatography (GC), liquid chromatography (LC) and capillary electrophoresis (CE) methodologies to tackle the diverse physicochemical properties of metabolites (polar, apolar, volatile, non-volatile, etc.). If methods are not available yet, we go back to the drawing board and develop the method from scratch and verify its performance.

  • We annotate metabolites using an in-house built accurate mass retention time (AMRT) or retention time locked (RTL) library which is continuously being updated with new entries.

  • We perform metabolomics experiments with project-specific QC samples guaranteeing validity of results.

  • We follow a rigorous and established game plan┬áthat helps define the right questions and determine which data will provide the answers.

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A few examples of projects we have supported in the past

  • Effect of genetic modifications on metabolite read-out (knock-out, knock-down, knock-in)

  • Discovery of biomarkers for bacterial infection in cystic fibrosis patients

  • Batch comparisons of complex raw materials and link with final product quality (e.g. fermentations)

  • Monitoring overall progress of chronic kidney disease

  • Early detection of parasite infections in humans

  • Studying the effect of drugs on cell metabolism