Finding your way through complex metabolic pathways
We provide a targeted and untargeted approach for a wide range of metabolites present in your samples using triple quadrupole, Q-TOF or Orbitrap mass spectrometry.
We include the necessary sample preparation steps prior to analysis, such as precipitation, liquid-liquid extraction, solid-phase extraction, derivatization.
We apply state-of-the-art gas chromatography (GC), liquid chromatography (LC) and capillary electrophoresis (CE) methodologies to tackle the diverse physicochemical properties of metabolites (polar, apolar, volatile, non-volatile, etc.). If methods are not available yet, we go back to the drawing board and develop the method from scratch and verify its performance.
We annotate metabolites using an in-house built accurate mass retention time (AMRT) or retention time locked (RTL) library which is continuously being updated with new entries.
We perform metabolomics experiments with project-specific QC samples guaranteeing validity of results.
We follow a rigorous and established game plan that helps define the right questions and determine which data will provide the answers.
A few examples of projects we have supported in the past
Effect of genetic modifications on metabolite read-out (knock-out, knock-down, knock-in)
Discovery of biomarkers for bacterial infection in cystic fibrosis patients
Batch comparisons of complex raw materials and link with final product quality (e.g. fermentations)
Monitoring overall progress of chronic kidney disease
Early detection of parasite infections in humans
Studying the effect of drugs on cell metabolism