PROTEOMICS
Accurately capturing the diversity in protein expression
We provide a bottom-up approach for proteins present in a diverse set of samples and perform (a) untargeted label-free proteomics using Orbitrap or Q-TOF mass spectrometry (broad coverage) or (b) targeted proteomics via stable isotopic dilution using triple quadrupole mass spectrometry (high sensitivity).
We include the necessary sample preparation steps prior to analysis, such as extraction, precipitation, enrichment, depletion and digestion.
We perform unidimensional high-resolution separations using pillar array columns and can readily add more chromatographic dimensions for ultimate sensitivity and coverage.
We use tailored software algorithms for protein identification and quantification.
We follow a rigorous and established game plan that helps define the right questions and determine which data will provide the answers.
A few examples of projects we have supported in the past
Identifying bait proteins interacting with prey proteins (studying protein-protein interactions)
Revealing pathogen-specific proteins & peptides in faeces & blood plasma
Classification of leaf types and assessing impact of processing steps
Quantifying transgenic proteins in artificial diets to prove environmental safety
Realtime detection of SARS-CoV-2 spike protein and nucleocapsid structural protein in nasal swabs & throat rinses
